| 1 | Make sure your sequence does not contain any characters apart from standard single letter amino acid codes and length greater than or equal to 30. |
| 2 | Only raw sequence format is accepted. FASTA format is not accepted. |
| 3 | Detailed results can be obtained by selecting "Detailed result" option, before submitting the query. |
| 4 | The confident score closer to 1 is considered to have higher proability that the query protein resides in the predicted location. |
| 5 | The ADA_AA, ADA_PSM, ADA_CTD, ADA_CPF, ADA_HYB, Signal Peptide and BLAST are different modules used in APSLAP. Refer the article for more explanation. |
| 6 | The values in the tables are vote (weight) of the particular subcellular localization for the corresponding modules. The localization with the maximum vote for the corresponding modules is bold faced. |
| 7 | Symbol "+" in the signal peptide module under secretory column and "-" in all other column indicates the presence of secretory signal peptide. Symbol "+" in all other column and "-" in secretory column indicates the absence of secretory signal peptide. |
| 8 | The values in the BLAST module indicates the percentage of similarity with the corresponding sub cellular localized protein. The value of 0.32 means a similarity of 32%, a value of 1 means a similarity of 100%, and a value of 0.045 means a similarity of 4.5%. |
| 9 | Function prediction result, if option selected before prediction, is self explanatory. Note: It may take a little more time if function prediction option is selected. |
| 10 | The result from the signalP prediction and BLAST alignment for location prediction and function prediction could be found at the bottom of the result page. |
For further queries contact Saravanan Vijayakumar (brsaran@bicpu.edu.in)